Journal: International Journal of Clinical and Experimental Pathology

Article Title: Degeneration of injured intervertebral discs affected by anterior longitudinal ligament injury in rabbits

doi:

Figure Lengend Snippet: Immunohistochemical examination of punctured IVDs and calculation of immunopositive cells with respect to TNF-α in different groups at 2 weeks and 8 weeks postoperatively ×400. A. The number of IVD cells gradually decreased and the percentage of immunopositive cells (marked by the arrow) gradually increased from sham group to Group C. B. The percentage of TNF-α-immunopositive cells in punctured IVDs in different groups and there was a significant difference between Group C and other groups (P<0.05) (*P<0.05).

Article Snippet: Sections were incubated with primary polyclonal anti-rabbit IL-1β antibody (Abcam, Shanghai, China; 1:200 dilution) and primary polyclonal anti-rabbit TNF-α antibody (Boster, Wuhan, China; 1:200 dilution), followed by secondary goat anti-rabbit antibody (Boster, Wuhan, China; 1:1 dilution) labelled with horseradish peroxidase.

Techniques: Immunohistochemical staining

Journal: Neural Regeneration Research

Article Title: Gastrodin inhibits neuroinflammation in rotenone-induced Parkinson's disease model rats ★

doi: 10.3969/j.issn.1673-5374.2012.05.001

Figure Lengend Snippet: Tumor necrosis factor-α staining in the midbrains of rats (immunohistochemical staining, light microscope, scale bars: 100 μm). Many dark brown-colored cells can be observed in the control rats (A). The number of positive cells was reduced in the model rats at 4 days (B) and 14 days (C). This was also the case in the gastrodin (D) and Madopar (E) groups at 14 days. The number of positive cells in the Madopar group at 28 days (F) was greater than that at 14 days (E).

Article Snippet: The sections were washed three times in distilled water three times (for 5 minutes each time), treated with 3% hydrogen peroxide for 10 minutes to block endogenous peroxidase, washed in distilled water and 0.01 M PBS, incubated with 5% normal goat serum for 30 minutes to inhibit non-specific binding, and incubated in primary rabbit anti-rat TNF-α monoclonal antibody (1: 50; Wuhan Boster Bio-engineering Co., Ltd, Wuhan, China) for 48 hours at 4°C.

Techniques: Staining, Immunohistochemical staining, Light Microscopy, Control

Journal: bioRxiv

Article Title: Proteolytic processing of LRP2 on RPE cells regulates BMP activity to control eye size and refractive error

doi: 10.1101/365817

Figure Lengend Snippet: A. Schematic showing tagging of a 432 amino acid region of Lrp2 with secreted eGFP at the N-terminus and mCherry at the C-terminus to monitor extracellular cleavage, referred to as seGFP-Lrp2 (ECD/ICD)-mCherry. B - D. HEK293T cells transfected with seGFP-Lrp2 (ECD/ICD)-mCherry were treated 24 hours after transfection with compounds and grown for another 24 hours before harvesting conditioned medium. eGFP-tagged domains cleaved from seGFP-Lrp2 (ECD/ICD)-mCherry were immobilized using Chromotek GFP-binding agarose before Western blotting and detection with JL-8 (anti-GFP antibody). E. When soluble eGFP-species levels from post-drug treatments were normalized to pre-treatment levels from the same culture plates, LY2811376-treatment appeared to reduce extracellular cleavage of seGFP-Lrp2 (ECD/ICD)-mCherry. Pixel intensity is expressed in arbitrary units based on ImageJ measurements of Western blot band densities.

Article Snippet: B - D. HEK293T cells transfected with seGFP-Lrp2 (ECD/ICD)-mCherry were treated 24 hours after transfection with compounds and grown for another 24 hours before harvesting conditioned medium. eGFP-tagged domains cleaved from seGFP-Lrp2 (ECD/ICD)-mCherry were immobilized using Chromotek GFP-binding agarose before Western blotting and detection with JL-8 (anti-GFP antibody).

Techniques: Transfection, Binding Assay, Western Blot

Journal: bioRxiv

Article Title: Proteolytic processing of LRP2 on RPE cells regulates BMP activity to control eye size and refractive error

doi: 10.1101/365817

Figure Lengend Snippet: A, B rpe65a:Lrp2 LDLA1 - eGFP or rpe65a:Lrp2 LDLA1 - ICD - eGFP plasmids were injected into BRE:dmKO2 reporter zebrafish and eye fluorescence measured at 4 dpf. C. Sclera/choroidal expression levels were unchanged from uninjected controls with Lrp2 LDLA1-ICD-eGFP (uninjected, 301 arbitrary units ± 173; Lrp2 LDLA1-ICD-eGFP, 320 arbitrary units ± 109), while Lrp2 LDLA1-eGFP expression led to significant reduction of Bmp activity (153 arbitrary units ± 73). Fluorescence levels were compared using one-way ANOVA with Tukey’s post-hoc test.

Article Snippet: B - D. HEK293T cells transfected with seGFP-Lrp2 (ECD/ICD)-mCherry were treated 24 hours after transfection with compounds and grown for another 24 hours before harvesting conditioned medium. eGFP-tagged domains cleaved from seGFP-Lrp2 (ECD/ICD)-mCherry were immobilized using Chromotek GFP-binding agarose before Western blotting and detection with JL-8 (anti-GFP antibody).

Techniques: Injection, Fluorescence, Expressing, Activity Assay